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α hcp1  (Cusabio)


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    Cusabio α hcp1
    α Hcp1, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+hcp1/Rabbit+anti-Escherichia+coli+dnaK+Polyclonal+Antibody/pm41723119-294-22-37
    Average 93 stars, based on 7 article reviews
    α hcp1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Control:

    Article Title: Effector conformational plasticity enables lineage-specific secretion via Hcp heterohexamers in gut symbionts
    Article Snippet: .. The contents of the gels were transferred to PVDF membranes (Millipore), blocked, and probed with primary antibodies α-Dnak (cytoplasmic control), α-Bte1 and α-Hcp1 (Rabbit α-Bte1 and α-Hcp1, this study, used at a dilution of 1:3000; Rabbit α-DnaK, Cusabio #CSB-PA633459HA01EGW, used at a dilution of 1:3000; Mouse α-HA, MBL #M180-3, used at a dilution of 1:2000), followed by horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit, MBL #458, diluted to 1:5000; goat anti-mouse, MBL #330, diluted to 1:5000). .. All protein bands were visualized using the M5 HiPer ECL Western HRP Substrate (Mei5 Biotechnology, MF074-05) and captured using the Tanon-5200 Chemiluminescent Imaging System (Tanon).

    Article Title: Effector conformational plasticity enables lineage-specific secretion via Hcp heterohexamers in gut symbionts.
    Article Snippet: .. The contents of the gels were transferred to PVDF membranes (Millipore), blocked, and probed with primary antibodies α-Dnak (cytoplasmic control), α-Bte1 and α-Hcp1 (Rabbit α-Bte1 and α-Hcp1, this study, used at a dilution of 1:3000; Rabbit α-DnaK, Cusabio #CSB-PA633459HA01EGW, used at a dilution of 1:3000; Mouse α-HA, MBL #M180-3, used at a dilution of 1:2000), followed by horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit, MBL #458, diluted to 1:5000; goat anti-mouse, MBL #330, diluted to 1:5000). .. All protein bands were visualized using the M5 HiPer ECL Western HRP Substrate (Mei5 Biotechnology, MF074-05) and captured using the Tanon-5200 Chemiluminescent Imaging System (Tanon).



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    α hcp1  (Cusabio)
    93
    Cusabio α hcp1
    α Hcp1, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+hcp1/Rabbit+anti-Escherichia+coli+dnaK+Polyclonal+Antibody/pm41723119-294-22-37
    Average 93 stars, based on 1 article reviews
    α hcp1 - by Bioz Stars, 2026-10
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    custom-made α-hcp1 antibody  (GenScript corporation)
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    GenScript corporation custom-made α-hcp1 antibody
    (A) VPA1263 co-precipitates with VgrG1. Immunoprecipitation of FLAG-tagged VPA1263 H837A from E. coli co-expressing the tail tube components Hcp1, VgrG1, or PAAR1 <t>(VP1415</t> AHH/AAA ) using α-FLAG antibodies. Tail tube components were detected using custom-made specific antibodies, collectively referred to as α-Tail tube. An asterisk denotes the expected size of PAAR1. (B) MIX is not required for VPA1263’s interaction with VgrG1. Immunoprecipitation of the indicated FLAG-tagged VPA1263 variants from E. coli co-expressing VgrG1 using either α-FLAG or α-VgrG1 antibodies. PoNi-FLAG was used as a negative control.
    Custom Made α Hcp1 Antibody, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+hcp1/rabbit+anti+hcp1+polyclonal+primary+antibodies/bio_rxiv__2022__05__04__489851-239-20-58
    Average 90 stars, based on 1 article reviews
    custom-made α-hcp1 antibody - by Bioz Stars, 2026-10
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    (A) VPA1263 co-precipitates with VgrG1. Immunoprecipitation of FLAG-tagged VPA1263 H837A from E. coli co-expressing the tail tube components Hcp1, VgrG1, or PAAR1 (VP1415 AHH/AAA ) using α-FLAG antibodies. Tail tube components were detected using custom-made specific antibodies, collectively referred to as α-Tail tube. An asterisk denotes the expected size of PAAR1. (B) MIX is not required for VPA1263’s interaction with VgrG1. Immunoprecipitation of the indicated FLAG-tagged VPA1263 variants from E. coli co-expressing VgrG1 using either α-FLAG or α-VgrG1 antibodies. PoNi-FLAG was used as a negative control.

    Journal: bioRxiv

    Article Title: The MIX domain is required for T6SS-mediated effector secretion

    doi: 10.1101/2022.05.04.489851

    Figure Lengend Snippet: (A) VPA1263 co-precipitates with VgrG1. Immunoprecipitation of FLAG-tagged VPA1263 H837A from E. coli co-expressing the tail tube components Hcp1, VgrG1, or PAAR1 (VP1415 AHH/AAA ) using α-FLAG antibodies. Tail tube components were detected using custom-made specific antibodies, collectively referred to as α-Tail tube. An asterisk denotes the expected size of PAAR1. (B) MIX is not required for VPA1263’s interaction with VgrG1. Immunoprecipitation of the indicated FLAG-tagged VPA1263 variants from E. coli co-expressing VgrG1 using either α-FLAG or α-VgrG1 antibodies. PoNi-FLAG was used as a negative control.

    Article Snippet: The following antibodies were used: DYKDDDDK Tag Antibody (α-FLAG), custom-made α-VgrG1 ( Li et al , 2017 ), and custom-made α-VP1415 and α-Hcp1 (polyclonal antibodies were raised in rabbit against the peptides CSKSTGDAGGDKKGV and CGTAGNDDWRAPREA, respectively; the cysteines at the end of the peptides were added during the synthesis process and are not found in the endogenous protein; GenScript).

    Techniques: Immunoprecipitation, Expressing, Negative Control